The problem

Standard analyses miss most of what's driving cell behavior.

Every analytical technology sees a slice. To design better media, better feed, or better recommendations, you need the whole molecular picture — not one slice.

The problem with slices.

Metabolomics captures small molecules. Proteomics captures protein signals. Standard RNA-seq analysis gives you differential expression tables and pathway lists. Each one shows a fragment of what's happening in your cells.

Miss the trace elements driving stress. Miss the growth factors shaping cell fate. Miss the metabolic pathways connecting expression to phenotype. Miss the mechanism, and you're guessing at recommendations from a partial view.

Auperon was built to unify those signals — mapping the entire molecular weight range of small and large molecules across your cells, then connecting what's measured to the reactome, to biological function, to actionable design.

  • Metabolomics aloneMetabolites without the transcriptional or regulatory context that produced them.
  • Proteomics aloneProteins without the pathways they modulate or the small-molecule cofactors they need.
  • RNA-seq analysis aloneExpression changes with no mapping to metabolic function or manufacturing outcome.
  • AuperonAll of it, unified — plus the digital twin, biomarker discovery, and modulator identification that translate the signal into action.
What we measure

The full molecular range. All at once.

Auperon maps the entire molecular weight range of large and small molecules for your cells — from trace elements to gene expression, and everything cells use in between to signal, metabolize, and respond.

A
Gene expression
Full transcriptome
B
Metabolic reactome
Pathway-level activity
C
Trace elements
Ion & metal utilization
D
Cytokines & growth factors
Signaling molecules
E
Cell stress markers
ROS, ER stress, UPR
F
Nutrient utilization
Macronutrients, cofactors, vitamins
G
Antioxidant enzymes
Redox & oxidative state
H
Chelating & buffering
Homeostasis molecules
I
Regulatory RNAs
Non-coding transcripts
J
Cell fate signatures
Pluripotency, lineage, differentiation
Platform architecture

Multi-omics in. Digital twin out.

Three integrated layers translate raw molecular signal into a working model of your cells and a ranked list of things you can do about it.

01 · INPUT

Deep cell characterization

Multi-omics: bulk RNA-seq at 50–150M paired-end depth (or your data format), plus proteomics and metabolomics where scoped.

What comes out: molecular signal across the entire measured weight range — expression, metabolic state, stress markers, cofactor availability.

02 · ANALYSIS

AI-driven translation

Informatics, deep learning, and digital twinning translate raw molecular signal into biological function — pathway-level insight, biomarker candidates, existing-molecule modulator identifications.

What comes out: mechanism-backed insight ranked by expected impact on your target phenotype.

03 · OUTPUT

Cellular digital twin

Your specific cells recapitulated in the cloud. Interactive dashboards, conversational query, and hypothesis simulation — built on your data, accessible after the engagement ends.

What comes out: a working model your team can interact with, ask questions of, and use to guide future work.

The digital twin

Your cells. In the cloud. Interactive.

The cellular digital twin is what separates Auperon from any static analysis. Once your data is loaded, you have a working model of your specific cells that you can interact with — not a report you read once and archive.

You get continued access after the engagement ends. Follow-up questions that don't require new sequencing are typically answerable directly from the existing twin — quick and inexpensive.

  • Explore physiology. Multi-omic dashboards across expression, metabolic pathways, biomarkers, and comparative analyses.
  • Simulate conditions. Test hypothetical changes and compare cell states without new bench experiments.
  • Query conversationally. ChatGPT-style interface built specifically on your results — ask what you actually want to know.
  • Compare across conditions. Cell states across conditions, timepoints, donors, or process variations, side by side.
What comes out

Biomarkers you can validate. Modulators you can source.

Auperon's outputs are engineered to be actionable — validatable independently, sourceable from existing supply, and defensible as IP.

Output · A

Biomarker discovery.

Novel biomarkers linked to specific CQAs — potency, viability, manufacturing success, therapy-relevant outcomes. Discovered from the multi-omic signal in your specific cells, not pulled from a generic panel.

Panel candidates you can validate independently and use for QC, comparability, process release, or regulatory documentation.

"We can predict manufacturing outcomes from starting material with a very high level of accuracy — even with a low number of samples and data that's somewhat low quality."
Output · B

Modulator identification.

Existing, characterized molecules that modulate the pathways driving your target phenotype. Novel IP through novel combinations — not new chemistry that needs to be qualified, characterized, or cleared.

Real, defensible IP with a cleaner CMC path. Your team can source these components from existing supply and go straight to validation.

"Ingredients that fall out of your normal formulations — we've found trace elements, growth factors, antioxidants, buffering molecules that hadn't been considered before for those specific cell types."
How it's different

Not just RNA-seq analysis. Not just a dashboard.

The most common question we get on intro calls is "how is this different from just running RNA-seq analysis?" Here's how — across the four comparisons that come up most often.

vs

Standard RNA-seq analysis

Standard tools give you differential expression tables and pathway enrichment lists. Auperon gives you mechanism-backed recommendations ranked by expected impact — with specific biomarker candidates and existing-molecule modulator identifications you can act on.

vs

Metabolomics only

Metabolomics catches metabolites. Auperon captures the full molecular universe — including the transcriptional and regulatory signals that connect metabolic state to cell fate, and the digital twin that ties all of it into function.

vs

Proteomics only

Proteomics catches proteins. Auperon connects protein signals to the pathways they modulate and the phenotypes they produce — so the output isn't "here's what's expressed," it's "here's what you can change."

vs

Pure software tools

Software tools give you dashboards. Auperon gives you dashboards backed by direct scientific-team access — mechanism-backed insight from PhD-level scientists who worked on your specific data, not a support queue.

Cells are talking

See what they've told other teams.

Case studies show the full workflow on real programs. Getting Started covers what to send us. Try It Now kicks off an engagement.